Project Description

TP02A: Modelling enteric hepatotropic virus infection: impact of the enteric stage on the outcome of liver infection

Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the leading causes of acute viral hepatitis in developing countries with poor sanitary conditions. Historically, as both viruses have been thought to only cause acute disease, interest in studying them has been low.
Stephanie Margarete Münchau, Rosalie Deutsch, Thomas Hielscher, Nora Heber, Beate Niesler, Megan Lynn Stanifer, Steeve Boulant. Hypoxic environment promotes barrier formation in human intestinal epithelial cells through regulation of miRNA-320a expression. (in press) MCB 2019
Pervolaraki K, Rastgou Talemi S, Albrecht D, Bormann F, Bamford C, Mendoza JL, Garcia KC, McLauchlan J, Höfer T, Stanifer ML, Boulant S. Differential induction of in-terferon stimulated genes between type I and type III interferons is independent of inter-feron receptor abundance. PLoS Pathog. 2018
Stanifer, M. L., Kischnick, C., Rippert, A., Albrecht, D., and Boulant, S. (2017) Reovirus inhibits interferon production by sequestering IRF3 into viral factories. Sci. Rep. 7, 10873
Pervolaraki, K., Stanifer, M. L., Münchau, S., Renn, L. A., Albrecht, D., Kurzhals, S., Senís, E., Grimm, D., Schröder-Braunstein, J., Rabin, R. L., and Boulant, S. (2017) Type I and Type III Interferons Display Different Dependency on Mitogen-Activated Protein Kinases to Mount an Antiviral State in the Human Gut. Front Immunol. 8, 459
Stanifer, M. L., Rippert, A., Kazakov, A., Willemsen, J., Bucher, D., Bender, S., Bartenschlager, R., Binder, M., and Boulant, S. (2016) Reovirus intermediate subviral particles constitute a strategy to infect intestinal epithelial cells by exploiting TGF-β dependent pro-survival signaling. Cell. Microbiol. 18, 1831–1845
Dao Thi VL, Debing Y, Wu X, Rice CM, Neyts J, Moradpour D*, and Gouttenoire J* (2016) Sofosbuvir inhibits Hepatitis E virus replication in vitro and results in an additive effect when combined with ribavirin. Gastroenterology. 150:82-85
Wu X*, Dao Thi VL*, Liu P, Takacs CN, Xiang K, Andrus L, Gouttenoire J, Moradpour D, and Rice CM (2018). Pan-Genotype Hepatitis E Virus Replication in Stem Cell-Derived Hepatocellular Systems. Gastroenterology. 154(3):663-674.e7
Wu X, Dao Thi VL, Huang Y, Billerbeck E, Saha D, Hoffmann HH, Wang Y, Vale Silva LA, Sarbanes S, Sun T, Andrus L, Quirk C, MacDonald MR, Schneider WM, An X, Rosenberg BR, and Rice CM (2018). Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 172(3):423-438.e25
Dao Thi VL, Wu X, and Rice CM (2018). Stem Cell-Derived Culture Models of Hepatitis E Virus Infection. Cold Spring Harb Perspect Med. pii: a03179
Gouttenoire J, Pollán A, Abrami L, Oechslin N, Mauron J, Matter M1, Oppliger J, Szkolnicka D, Dao Thi VL, van der Goot FG, Moradpour D. (2018) Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion PLoS Pathog. 2018 Dec 10;14(12):e1007471. doi: 10.1371/journal.ppat.1007471